BIOLADDER® Dual Color Prestained Protein marker

500 ul, 2.6mg/ml

 

 

 

 

文字方塊: 4-20 % SDS - PAEG

 

 

 

 

Ready-to-use Premix in 500 μl gel loading buffer, Dual ColorApplicable Applications:

Description:

Prestained Protein Marker II, middle molecular weight

range, resolved to 8 sharp bands when analyzed by

SDS-PAGE, is a mixture of purified proteins covalently

coupled to blue and orange chromophore. It consists of 8

proteins ranging in apparent molecular weight from

approximately 15 kD to 150 kD. Prestained Protein Marker

II is supplied in gel loading buffer and ready-to-use.

Applications:

Molecular weight estimation

Monitoring protein transfer efficiency onto membranes

during Western blotting

Monitoring electrophoresis separation

Recommended Usage:

Thaw the Marker either at room temperature or 37-40for 1 minute if any solids

was precipitated at -20.

After a quick spin, load directly onto a gel:

For blotting: load 3 μl for mini-gels and 6 μl for full length gels.

For visualizing during electrophoresis: load 5 μl for mini-gels and 10 μl for full

length gels.

Quality Control:

Tested in SDS-polyacrylamide gel electrophoresis and Western blotting

Contents:

T0.2-0.8 mg/ml of each protein

70 mM Tris-HCl (pH 6.8)

33mM NaCl

1 mM EDTA

2% SDS

5 % 2-mercaptoethanol

10% glycerol

Storage Condition:

Aliquot to prevent freeze and thaw cycles.

Shelf Life: One year at -20 .

Note:

This product is for research use only.

文字方塊: Product NO.
specification
 
BL1021
BIOLADDER® Dual Color Prestained Protein marker
 
 500 ul, 2.6mg/ml
 
 
 
 
 
 
 
 
 
 

 

 

 

   
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