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Lambda DNA /Hind III
Markers
are prepared by digesting Lambda DNA with
Hind III, followed by heat-inactivation
of the enzyme. The DNA fragments are then
ethanol-precipitated and resuspended in the
storage buffer. The
Marker is suitable for size and quantitative
of linear double-stranded DNA fragments and
the fragments is contains
23,130;
9,416;
6,557; 4,361;
2,322;
2,027; 564, 125
Storage Buffer:
10mM Tris-HCl (pH
8.0),
1mM EDTA.
Concentration:
0.2-0.5µg/µl.
Number of Bands:
8.
Storage
-20°C.
Attention!
The cohesive ends of fragments 1 and 4 may
cause formation of extra band 27491 bp. The
fragments may be
separated by heating to 65°C
for 3 minutes
before loading the sample on the gel.
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