2X PCR Master Mix

5.25 ml ( 1.75 ml x 3 )

 

 

 

 

Violet PCR Master is optimized mixture contain of Taq enzyme, reaction buffer, dNTP, enhancer. Violet PCR master is designed to allow the user for quick ,easy preparation and ready loading of reaction mixture. The Violet PCR Master can  be amplification PCR products up to 5 kb and the products can be directly cloning into T-vector.

Applications :

1.     Standard PCR of genome, viral and plasmid DNA

2.     PCR for Cloning

3.     High throughput PCR

Features :

1.     Competitive price with other supplier

2.     Highly activity, sensitivity and thermostability

3.     The longest products is 5-6 Kb

Storage Condition : shipped and stored at 4 ℃

Mg2+ concentration: 3mM

 

 

 

 

 

 

 

 

 

文字方塊: General Reaction :
Composition
Volume/Rxn (50 ul)
2 X master mix
25 ul
Template DNA
< 1 ug
Primer F 
0.2-1 uM
Primer R
0.2-1 uM
dd Water
Up to 50ul
 Recommended thermal cycling condition :
Step
Temperature
Time
Cycle
Initial denaturation
94-96oC
0.5-2mins
1
Denaturation
94-96oC
0.2-2mins
15-30
Annealing
37-70
0.2-2mins
Extension
68-75
1min/1kb
Final extension
68-75
1-10mins
1
 

 

 

 

 

 

   
Copyright 2002 BASIC LIFE SCIENCE, INC. All Rights Reserved
文字方塊: Product NO.
specification
 
VIO-T809
2X PCR Master Mix
5.25 ml ( 1.75 ml x 3 )