2 X  Redy  PCR Master Mix

5.25ml (1.75ml×3)

 

 

 

 

 

文字方塊: Fig : 
Comparison activity of amplified the PCR
products  contain :200bp, 500bp,700bp,
1000bp,1500bp and 2500bp
 

 

 

 

Redy Master is optimized mixture contain of Taq enzyme, reaction buffer, dNTP, enhancer and red dye as 2-fold concentration. Redy master is designed to allow the user for quick ,easy preparation and ready loading of reaction mixture. The Redy Master can  be amplification PCR products up to 5 kb and the products can be directly cloning into T-vector.

Applications :

1. Standard PCR of genome, viral and plasmid DNA

2. PCR for Cloning

3. High throughput PCR

Features :

1.Competitive price with other supplier

2.Highly activity, sensitivity and thermostability

3.The longest products is 5-6 Kb

Storage Condition shipped and stored at 4

Mg2+ concentration: 3mM

The red dye non effect activity of Taq DNA polymerase

文字方塊: General Reaction :
Composition
Volume/Rxn (50 ul)
2 X master mix
25 ul
Template DNA
< 1 ug
Primer F
0.2-1 uM
Primer R
0.2-1 uM
dd Water
Up to 50ul
 
Step
Temperature
Time
Cycle
Initial denaturation
94-96oC
0.5-2mins
1
Denaturation
94-96oC
0.2-2mins
15-30
Annealing
37-70
0.2-2mins
Extension
68-75
1min/1kb
Final extension
68-75
1-10mins
1
Recommended thermal cycling condition :
 

 

 

 

 

 

 

 

 

 

 

 

文字方塊: Product NO.
specification
 
BLT809
2 X Read PCR Master Mix
5.25ml (1.75ml×2)
 

 

 

 

 

   
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