The Coomassie Plus Reagent is a quick and
ready-to-use coomassie-binding, colorimetric method for
total protein quantitation.When coomassie dye binds
protein in an acidic medium, an immediate shift in
absorption maximum occurs from 465 nm to 595 nm with a
concomitant color change from brown to blue
Note: Protein concentrations should be
estimated by reference to absorbance measurements
obtained for a series of standard protein dilutions,
which are assayed alongside the unknown samples.
Excellent protein standards, including Albumin (BSA)
reagent is ready to use. No mixing or dilution required.
Color development is rapid. Only a five minute
incubation and then the sample is read a 595 nm.
Reducing sugars and reducing substances along with
thiols do not interfere with this reagent.
Reagent is suitable for micro (1- 10 mg/ml) and standard
(50-1400 mg/ml) assays
◎ Can be used in microwell plate