The Coomassie Plus Reagent is a quick and ready-to-use coomassie-binding, colorimetric method for total protein quantitation.When coomassie dye binds protein in an acidic medium, an immediate shift in absorption maximum occurs from 465 nm to 595 nm with a concomitant color change from brown to blue       

Note: Protein concentrations should be estimated by reference to absorbance measurements obtained for a series of standard protein dilutions, which are assayed alongside the unknown samples. Excellent protein standards, including Albumin (BSA)

Benefits:

◎    The reagent is ready to use. No mixing or dilution required.

◎    Color development is rapid. Only a five minute incubation and then the sample is read a 595 nm.

◎    Reducing sugars and reducing substances along with thiols do not interfere with this reagent.

◎    Reagent is suitable for micro (1- 10 mg/ml) and standard (50-1400 mg/ml) assays

◎    Can be used in microwell plate assays