Hot start Taq DNA Polymerase for qPCR is
designed for Real-Time PCR and Hot-start PCR. A special
inhibition the reaction at room temperature until after
the first denaturation step. This prevents primer-dimers
and other artefacts.
The enzyme is a thermostable DNA polymerase that
possesses a 5’→3’ polymerase activity and a
double-stranded specific 5’→3’ exonuclease activity. The
enzyme consists of a single polypeptide with a molecular
weight of 94kDa.
and real time PCR
of complex genomic and cDNA templates
primer-dimers and other artefacts; inactive at room
activation time for real time PCR
Recommended thermal cycling condition: