|
Ultrase HiFi DNA polymerase is
thermostable enzyme formulation specifically developed
for length up to 30kb and low error rate PCR product.
Ultrase HiFi DNA polymerase synthesizes higher yields
and accuracy of product from genomic DNA, cDNA,
bacterial cultures. It is contain a 2.5 hours half life
at 96oC and easy to amplify PCR product at G-C rich and
secondary structure.
Reaction Buffer (10x) with MgCl2:
25 mM
Store Condition :
-20℃
Recommended thermal cycling condition:
|
Step |
Temperature |
Time |
Cycle |
|
Initial denaturation |
94-96oC |
0.5-2mins |
1 |
|
Denaturation |
94-96oC |
0.2-2mins |
|
|
Annealing |
37-70 |
0.2-2mins |
15-30 |
|
Extension |
68-75 |
1min/1kb |
|
|
Final extension |
68-75 |
1-10mins |
1 |
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