|
Ultrase TS DNA polymerase is a
thermostable enzyme formulation specifically developed
for maximizing and low error rate PCR product. Ultrase
TS DNA polymerase synthesizes higher yields of product
from genomic DNA, cDNA, bacterial cultures. It is
contain a 2.5 hours half life at 96℃
and easy to amplify PCR product at G-C rich and
secondary structure.
Reaction Buffer (10x) with MgCl2:
25 mM
Store Condition :
-20℃
Recommended thermal cycling condition:
|
Step |
Temperature |
Time |
Cycle |
|
Initial denaturation |
94-96oC |
0.5-2mins |
1 |
|
Denaturation |
94-96oC |
0.2-2mins |
|
|
Annealing |
37-70 |
0.2-2mins |
20-30 |
|
Extension |
68-75 |
30sec/1kb |
|
|
Final extension |
68-75 |
1-10mins |
1 |
|
