Figure: Map and
Sequence reference points of the yT&A cloning vector
*Before the insert is
incorporated into the yT&A cloning vector, there is
only one HindIII site and no Bg/II site. After the
incorporation, the T and A nucleotide on the the
insert will complement the sequence on the vector
and generate these two new site. This merit of yT&A
vector makes cloning more economical and convenient.