T4 DNA Ligase
T4 DNA Ligase catalyzes the formation of
a phosphodiester bonds between 5'-phosphate and
3'-hydroxyl ends in double strand DNA or RNA with blunt
or cohesive-end termini.
double-stranded DNA with cohesive or blunt termini and
Storage Buffer :20mM
Tris-HCl (pH 7.5), 1mM DTT, 50mM KCl, 0.1mM EDTA and 50%
5X Ligation Buffer :contain
with Tris-HCl, MgCl2, DTT, ATP (pH 7.8 at 25oC) and PEG.
heating at 65oC for 10min.
Unit definition :0.01
Weiss unit is defined as the amount of enzyme required
to catalyze the ligation of greater than 95% of the Hind
III fragments of 1 ug of Lambda DNA at 16°C in 20
detectable nuclease activities.
Additional reference :Polyethylene
glycol (PEG) greatly increases the rate of ligation of