T4 DNA Ligase

2.5u/ul；100U/500U

T4  DNA Ligase catalyzes the formation of a phosphodiester bonds between 5'-phosphate and 3'-hydroxyl ends in double strand DNA or RNA with blunt or cohesive-end termini.

Applications :Joining double-stranded DNA with cohesive or blunt termini and 3’T-A overhangs.

Storage Buffer :20mM Tris-HCl (pH 7.5), 1mM DTT, 50mM KCl, 0.1mM EDTA and 50% glycerol.

5X Ligation Buffer :contain with Tris-HCl, MgCl2, DTT, ATP (pH 7.8 at 25oC) and PEG.

Inactivation :By heating at 65oC for 10min.

Unit definition :0.01 Weiss unit is defined as the amount of enzyme required to catalyze the ligation of greater than 95% of the Hind III fragments of 1 ug of Lambda DNA at 16°C in 20 minutes.

Purity :No detectable nuclease activities.

Additional reference :Polyethylene glycol (PEG) greatly increases the rate of ligation of blunt-ended DNA.